Ameliorative Effect of Vitamin C And E Against The Toxicity of Nitrogen Dioxide Gas on Clotting Factors in Albino Rats

Rafat Khan, Asha Agarwal

Abstract


In the present study, ameliorative effect of vitamin C and E against the nitrogen dioxide gas toxicity on blood plasma factors   Fibrinogen deficiency plasma (FDP), Prothombin time(PT) , Partial thromboplastin time (PTT), Activated partial thromboplastin time (APTT) ,Thrombin time (TT) and calcium ions(Ca++) were studied  in both the sexes of albino rats.A significant  increase in plasma fibrinogen deficiency plasma level (FDP) and a significant decrease in protrombin time (PT), partial thromboplastin time (PTT), activated partial thromboplastin time (APTT), thrombin time  (TT) and calcium ions  have been reported  in both the sexs of albino rats after exposure  to 50ppm  nitrogen dioxide gas  for 45 days for one hour per day. A significant variation in plasma clotting factors is accompanied with inflammation and oxidative stress induced by toxic inhalation of nitrogen dioxide gas. Rats of equal size and weight (150-200gm) were kept in standard laboratory conditions and divided into three groups containing ten rats in each   group. GroupI is control set, group II and group III are experimental sets. control set I was unexposed, set II  was exposed to  50ppm  nitrogen dioxide gas alone, while set III was exposed to 50ppm nitrogen dioxide gas along -with supplementation of antioxidants vitamin C (5mg/rat) and vitamin E (2.5mg/rat) for one hour per day for 45 days. Results of present study shows that experimental set II shows disorders in clotting factors induced by nitrogen dioxide gas in comparison to control set I, while set III shows mitigation in disorder in clotting factors by supplementation of antioxidants vitamin C and E in comparison to experimental set II.Present study Reveal that toxicity of nitrogen dioxide gas have been attenuated by supplementation of vitamin C and E due to antioxidant defence mechanism in both the sexes of albino rats.


Full Text:

PDF

References


Achuba, F.I. 2005. Effect of vitamin C and E intake on blood lipid peroxidation superoxide dismutase and catalase activities in rabbit fed petroleum contaminated diet. European Journal of Scientific research, 12(1); 1-8.

Anonymous 1996. Antioxidant depletion, lipid peroxiation and impairment of calcium transport induced by air blast overpressure in rat lungs. Exper. Lung. Res. (USA), 22(2): 179-200.

Baccarelli, A. Zanobetti, I. Martinelli, P. Grillo, L. Hou, S. Giaconins, M. Bonzini, G. Lanzani, P.M. Mannucci, and P.A. Beriazzi 2007. Effects of exposure to air pollution on blood coagulation. J. Thromb. Haemost, 5 (2): 252-60.

Beyer, R.E. 1994. The role of ascorbate in antioxidants protection of biomolecules: interaction with vitamin C and coenzyme Q. J. Bioenergy Biomemb., 26: 349-358.

Blomberg, A., H. Tornqvisit, L. Desmytes, V. Deneys and C. Her mans 2005. Exposure to diesel exhaust nanoparticles does not induce blood hyper coagulability in an at risk population. J. Thromb. Haemost., 3: 2103-2105.

Brook, R.D., B. Franklin, W. Cascio, Y. Hong and I. Tager 2004. Air pollution and cardiovascular diseases. Circulation, 109: 2655-2671.

Biggs, R. 1972. Human blood coagulation, Haemostasis and thrombosis, Blackwell scientific publication, Oxford, England.

Burton, A. 2007. Particulate matter: the clot thickness: new clues to PM action. Environ. Health Perspect, 115(12): A580-A581.

Chen, H. and A.L. Tappel 1995. Protection by vitamin E, selenium, trolex C, ascorbic acid, palmitate, acetyl, coenzyme Q, coenzyme Q10, beta-carotene, canthovantin and catechin against oxidative damage to red blood cells and tissue in vivo. Free Radical. Biol. Med., 18: 949-953.

Colman, R.W., J. Hirsh, V.J. Marder, E.W. Saltzman 1994. Haemostasis and thrombosis- Basic principles and clinical practice. J.B. Lippincott, pp. 1197-1206.

Levaggi, D.A., S. Wayman and M. Feldstein 1972. Method for the production of nitric oxide. Environ. Sci. Technol., 6: 250.

Lass, A. and R.S. Sohal 2000. Effect of coenzyme Q10 and alpha-tocopherol content of mitochondria on production of superoxide anion radicals. FASEB. J., 14: 87-94.

Mutlu M. Gokhar, D. Green, A. Bellneyer, C.M. Baker, Burgess, N. Rajamanna, J.W. Christnan, N. Foiles, D.W. Kamp, A.J. Ghio, N.S. Chandel, D.A. Dean, J.I. Sznajder and G.R. Scott Budinfer 2007b.

Ambient particulate matter accelerates coagulation via an IL-6-dependent pathway. J. Clin. Invest., 117: 2952-2961.

Nel, A. 2005. Atmosphere. Air pollution related illness: effects of particles. Science, 308: 804-806.

Nemmar, A., et al. 2003.

Diesel exhaust particle in lung acutely enhance experimental peripheral thrombosis. Circulation, 107: 1201-1208.

Pekkanen, J., Breenes, S.J., Anderson, H.R. 2000. Daily concentration of air pollution and plasma fibrinogen in London. J. occup. Environ. Med., 57: 818-822.

Reed, M., Gigliatti, J. Mcdonald, J. Scagrave S. Seilkop, J. Mauderly 2004. Health effects of subchronic exposure to environment levels of diesel exhaust. Inhalation Toxicology, 16: 177-193.

Ruckerl, R., et al. 2006. Air pollution and markers of inflameltion and coagulation in patients with coronary heart disease. Am. J. Respir. Crit. Care. Med., 173: 432-441.

Rivero, D.H.R.F., S.R.C. Soares, G.L. Filho, M. Saiki, J.L. Godleski, L. Antonagelo, M. Dolhnikoff and P.H.N. Saldiva 2005. Acute cardiopulmonary alterations induced by fine particulate matter. Tox. Sci. Advance Acess, 85(2): 898-905.

Saltzman, B.E. 1954. Colorimetric microdetermination of nitrogen dioxide in the atmosphere. Anal. Chem., 26 : 1949-1950.

Schwela D., 2000. Air pollution and health in urban areas. Jan-Jun Rev. Environ Hlth., 16: 20-25.

Tizard, I.R. 1988. The inflammation Immunology an introdsuction. Saunders College publishing, pp. 431-436.


Refbacks

  • There are currently no refbacks.